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Biomedical Center for Microscopy
Vrazov trg 2
Ljubljana

telephone: 386 1 5437680
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Biomedical Center for Microscopy


24.09.2018 2:30

Biomedical Center for Microscopy

 

Biomedical Center for Microscopy

Head of the Infrastructural Center: Prof. Dr. Peter Veranič

 

The scope of the infrastructural center:

Understanding how biological processes work in organisms is based on the knowledge of these processes at the cellular and molecular level. This means that it is necessary to know the microenvironment, wherein these processes take place. New active substances used in medicine, pharmacy and food and other branches of industry are in the early stages of examination usually tested on cells. Microscopy with its numerous supporting methods give the most accurate answer to the impact of these substances on survival and function of the cells. In addition, the methods of microscopy are indispensable in certain pathological conditions, where the diagnosis is based on deviation from the normal structure of the cells or by the presence of pathogens.

 

Recently, there is a very rapid development of microscopy techniques, offering the possibility of studying living cells, i.e. monitoring the dynamic processes in real time, as well as methods of labelling molecules within cells. The two basic branches of microscopy, light and electron microscopy, are increasingly converging and complementary. Connection between the two arms of the microscopy, the so-called correlative microscopy, combines the study of live cells by light microscopy and the high-resolution of electron microscopy.

 

 

The methods used at the Biomedical Center for Microscopy

Light and electron microscopy belong to the basic methods for the study of biological processes at the cellular level, which allow determining the locations where these processes take place. Methods of microscopy are rapidly evolving and thus, offer new opportunities to study living cells and provide posibility of tracking individual molecules at the cellular level or determine location of these molecules in the intracellular structures.

 

Transmission electron microscopy (TEM)

 

Labeling of molecules at the electron microscopic level

 

Scanning electron microscopy (SEM)

 

Freezefracture replica immunolabeling (FRIL)

 

Electron tomography (three-dimensional reconstruction at the TEM level)

 

Immunofluorescence microscopy with optical sectioning (confocal microscopy, TIRF)

 

Microscopy of living cells and timelapse microscopy

 

Correlative microscopy (link light and electron microscopy)

 

Cell culturing

 

Production of nanoparticles

 

 

Equipment of the Biomedical Center for Microscopy

The main equipment of the Biomedical Center for Microscopy comprising transmission and scanning electron microscopes, together with the devices for the implementation of the latest methods of immunolabelling of cellular molecules, the negative contrasting, kriometods, correlative microscopy methods, FRIL and electronic tomography. Another assembly of equipment of the center consists of  classical light and fluorescence microscopy which methods are indispensable in any molecular biological or cell biological research and in diagnostics. There is also the equipment required for optical sectioning of fluorescently labeled samples and for qualitative and quantitative analysis of labelied samples that include  confocal microscopes, a TIRF microscope and a microscope with structural illumination. The equipment is suitable also for the combination of light and electron microscopy, the so-called correlative microscopy, that combines the advantages of light and electron microscopy, in particular the study of dynamic processes in living cells and resolutions down to 1 nm. Cell culturing and production of nanoparticles give the Biomedical Center of Microscopy excellent opportunities for the specific targeting of the cancerous or pathogen-infected cells with active substances. A characteristic feature of the Center for Biomedical Microscopy is its interdisciplinarity, which is crucial for successful work and optimum utilization of equipment and the development of methods.

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Vrazov trg 2, 1104 Ljubljana, Slovenija -- telefon: +386 1 543 7700, faks: +386 1 543 7701 -- e-pošta:

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