Spletna stran Laboratorija LN-MCP
Vodja laboratorija: Robert Zorec
The longterm program of the Laboratory of Neuroendocrinology, is to study molecular mechanisms of regulated exocytotic hormone secretion. The function of a single cell is studied by electrophysiological techniques of patch-clamping, where the secretory activity is monitored by changes in membrane capacitance. Since cytosolic calcium activity is the trigger of exocytosis in excitable cells, fluorescence (photometric and imaging) methods are used to measure changes in cytosolic calcium. Caged calcium photolysis is used to produce a step change in cytosolic calcium. A method was developed to quantify the interaction between a single secretory granule and plasmalemma, detected as discrete steps in membrane capacitance (femtofarad steps). A combination of molecular biology and electrophysiology approach was adopted to inhibit expression of monomeric GTP-binding proteins. Confocal microscopy is used to study molecular mechanisms of regulated exocytosis in endocrine cells, astrocytes and photoreceptors. We also use confocal microscopy to evaluate our bioengeneering products.