Laboratory for extracellular vesicle research
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Equipment
We also provide commercial analytical services and project-based support for EV research. Please contact us for further information ().
Optima XPN-80 Ultracentrifuge (Beckman Coulter)
Optima XPN-80 (Max g-Force: 548,300 ×g)
Equipped with rotors:
Type 50.2 Ti
SW 41 Ti Swinging-Bucket
Optima MAX-XP (Max g-Force: 1,019,000 ×g)
Equipped with rotors:
MLA-50
MLA-55
MLS-50 Swinging-Bucket
TLA-55
Responsible person: Samuel Žvanut
(including for access to Optima MAX-XP Ultracentrifuge (Beckman Coulter) at IBKMG)
Flow Nanoanalyzer – Nano-flow cytometer (NanoFCM)
Nano-flow cytometer for single particle (e.g. EVs) characterization, including concentration, size distribution, fluorescence-based subpopulation analysis, and quantification of fluorophores per particle.
Responsible person: Maja Vodušek
NanoSight NS300 with Automated Sample Assistant (Malvern Panalytical)
Nanoparticle Tracking Analysis (NTA) system for characterization of heterogeneous nanoparticles (e.g. EVs), including particle concentration and size distribution.
Responsible person: Teja Lavrin
Axio Imager M2 Motorized Upright Microscope with Camera Axiocam 305 mono (Zeiss)
Equipped with the following filter sets:
Filter set 38 HE (EX: BP 470/40, EM: BP 525/50)
Filter set 43 HE (EX: BP 550/25, EM: BP 605/70)
Filter set 49 (EX: G 365, EM: BP 445/50)
Filter Set 64 HE (EX: BP 587/25, EM: BP 647/70)
Responsible person: Teja Lavrin
Synergy 2 Microplate Spectrophotometer (Biotek)
Works with standard 6- to 384-well plates and Take3 microvolume plates.
UV-Vis absorbance (200 to 999 nm)
Fluorescene (EX: 360/40, 485/20, 530/25; EM: 460/40, 528/20)
Luminiscence (detection limit: 50 amol ATP/well)
Responsible person: Samuel Žvanut
Agilent BioTek 50 TS microplate washer (Agilent)
96-well plate washer equipped with biomagnetic separation and vacuum filtration modules.
Responsible person: Samuel Žvanut